Part:BBa_J61000:Design

chloramphenicol resistance cassette

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

N/A

Source

PCR ca1016F/R on pKD3 (934 bp, EcoRI/SpeI)

Sub into pSB1A2-I13522 (EcoRI/SpeI)

Products are Bca1015 and Bca1016

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ca1016F Forward EcoRI to biobrick CAT of pKD3

gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg

ca1016R Reverse SpeI to biobrick CAT of pKD3

ccgctactagtggcgcgcctacctgtgacgg